The reaction of acetyl-coenzyme A with choline acetyltransferase

Choline acetyltransferase. Evidence for an acetyl-enzyme reaction intermediate.

Choline acetyltransferase: reversible inhibition by bromoacetyl coenzyme A and bromoacetylcholine.

Choline acetyltransferase (EC 2.3.1.6) catalyzes the following reversible reaction: acetyl coenzyme A + choline acetylcholine + coenzyme A. Bromoacetyl-CoA and bromoacetylcholine are potent inhibitors of the bovine brain enzyme. Although the enzyme is inactive immediately following Sephadex gel filtration to remove unreacted inhibitor, enzyme activity returns to normal in 1 day at 0". When the ...

Studies of the Acetyl Coenzyme A Synthetase Reaction

Acetyl coenzyme A synthetase, which catalyzes the biosynthesis of acetyl coenzyme A from acetat,e, adenosine triphosphate, and coenzyme A, has previously been obtained in highly purified form from bovine heart mitochondria (1). The enzyme appeared to be fairly homogeneous because only a single protein peak was detected in two chromatographic systems and the pattern observed in the analytical ul...

Evidence for presence of an arginine residue in the coenzyme A binding site of choline acetyltransferase.

Choline acetyltransferase (acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6) may be inactivated by arginine-specific reagents such as butanedione, phenylglyoxal, and camphorquinone-10-sulfonic acid. The enantiomers of the latter compound were prepared, but inactivation was not stereospecific. Protection against inactivation by the arginine-specific reagents was provided by CoA and, to a lesse...

Long Chain Base-Acetyl Coenzyme A Acetyltransferase from the Microsomes of Hansenula ciferri

Microsomes of the yeast, Hansenula ciferri NRRL Y-1031, F-60-10, contain an enzyme (long chain base-acetyl coenzyme A acetyltransferase, EC 2.3 . 1) which catalyzes the transfer of the acetyl moiety of acetyl coenzyme A to both the amino and hydroxyl groups of the sphingosine bases; a separation of these two transfer reactions could not be achieved. The enzyme also catalyzed an acetyl transfer ...